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HA-MBP-TEVpCS-ADGRL3 CTF-FLAG
(Plasmid #217708)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217708 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 217708-D50 50 μg of DNA in Tris buffer $495
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DNA 217708-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pFuse-hIgG1-Fc2
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ADGRL3
  • Alt name
    LPHN3
  • Species
    H. sapiens (human)
  • Mutation
    Deletion of residues 1-841
  • Entrez Gene
    ADGRL3 (a.k.a. CIRL3, CL3, LEC3, LPHN3)
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-MBP-TEVpCS-ADGRL3 CTF-FLAG was a gift from Stephen Blacklow (Addgene plasmid # 217708 ; http://n2t.net/addgene:217708 ; RRID:Addgene_217708)

  • For your References section:

    Heterogeneity of tethered agonist signaling in adhesion G protein-coupled receptors. Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse AC, Blacklow SC. Cell Chem Biol. 2024 Aug 15;31(8):1542-1553.e4. doi: 10.1016/j.chembiol.2024.03.004. Epub 2024 Apr 11. 10.1016/j.chembiol.2024.03.004 PubMed 38608683
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