pES1004
(Plasmid #21790)

• Depositing Lab: Richard Yu
• Publication: Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Backbone

• Vector backbone: pRS406
• Backbone size w/o insert (bp): 4300
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kss1-as2-trunc
• Alt name: Kss1
• Alt name: Kss1-as2
• Alt name: Kss1-as
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1486
• Mutation: deleted bases 1-14 from open reading frame E94A mutation 401 3' UTR / terminator included
• GenBank ID: NC_001139
• Entrez Gene: KSS1 (a.k.a. YGR040W)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Eduard Serra ()

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry