SFFV-miR-SCR-AG
(Plasmid
#218479)
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PurposeLentiviral miRNA overexpression
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 218479 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneSFFV
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namescrambled miRNA
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SpeciesH. sapiens (human)
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Insert Size (bp)605
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Mutationscrambled seed
- Promoter SFFV
Cloning Information
- Cloning method Other
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Genomic miR-142 sequence with a scrambled seed, inserted with In-Fusion.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SFFV-miR-SCR-AG was a gift from Filipe Pereira (Addgene plasmid # 218479 ; http://n2t.net/addgene:218479 ; RRID:Addgene_218479)