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ATF4 11: 5’ATF4.uORF2.GFP
(Plasmid #21858)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21858 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4733
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ATF4
  • Alt name
    upstream ORF2 of ATF4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    340
  • Mutation
    upstream ORF2 (uORF) of ATF4 fused to EGFP
  • Entrez Gene
    Atf4 (a.k.a. Atf-4, C/ATF, CREB-2, CREB2, TAXREB67)
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindII (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

These pEGFP-based plasmids have NEOr marker (and not the conventional AMPr marker). They should be propagated in the presence of 30mcg/ml kanamycin (and not on ampicillin)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ATF4 11: 5’ATF4.uORF2.GFP was a gift from David Ron (Addgene plasmid # 21858 ; http://n2t.net/addgene:21858 ; RRID:Addgene_21858)
  • For your References section:

    Translation reinitiation at alternative open reading frames regulates gene expression in an integrated stress response. Lu PD, Harding HP, Ron D. J Cell Biol. 2004 Oct 11. 167(1):27-33. 10.1083/jcb.200408003 PubMed 15479734