Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

ATF4 11: 5’ATF4.uORF2.GFP
(Plasmid #21858)


Item Catalog # Description Quantity Price (USD)
Plasmid 21858 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4733
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
    upstream ORF2 of ATF4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
    upstream ORF2 (uORF) of ATF4 fused to EGFP
  • Entrez Gene
    Atf4 (a.k.a. Atf-4, C/ATF, CREB-2, CREB2, TAXREB67)
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindII (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

These pEGFP-based plasmids have NEOr marker (and not the conventional AMPr marker). They should be propagated in the presence of 30mcg/ml kanamycin (and not on ampicillin)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ATF4 11: 5’ATF4.uORF2.GFP was a gift from David Ron (Addgene plasmid # 21858 ; ; RRID:Addgene_21858)
  • For your References section:

    Translation reinitiation at alternative open reading frames regulates gene expression in an integrated stress response. Lu PD, Harding HP, Ron D. J Cell Biol. 2004 Oct 11. 167(1):27-33. 10.1083/jcb.200408003 PubMed 15479734