|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21905||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7558
Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
Alt nameshRNA GFP
Insert Size (bp)55
- Cloning method Restriction Enzyme
- 5′ cloning site HpaI (destroyed during cloning)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer cacagacttgtgggagaagc (Common Sequencing Primers)
GFP RNAi sequence - 5'-ACAGCCACAACGTCTATAT
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSicoR-mCh-GFPi was a gift from Miguel Ramalho-Santos (Addgene plasmid # 21905 ; http://n2t.net/addgene:21905 ; RRID:Addgene_21905)
For your References section:Chd1 regulates open chromatin and pluripotency of embryonic stem cells. Gaspar-Maia A, Alajem A, Polesso F, Sridharan R, Mason MJ, Heidersbach A, Ramalho-Santos J, McManus MT, Plath K, Meshorer E, Ramalho-Santos M. Nature. 2009 Jul 8. ():. 10.1038/nature08212 PubMed 19587682