Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21925||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberLow Copy
Gene/Insert namehnRNP H1
Alt namehnRNP H
SpeciesH. sapiens (human)
Insert Size (bp)1353
Entrez GeneHNRNPH1 (a.k.a. HNRPH, HNRPH1, hnRNPH)
/ Fusion Protein
- 3 Flags (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Note that the 3' end of this insert is extremely difficult to sequence for unknown reasons.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p3x-FLAG-hnRNPH1 was a gift from Mariano Garcia-Blanco (Addgene plasmid # 21925 ; http://n2t.net/addgene:21925 ; RRID:Addgene_21925)
For your References section:hnRNP H and hnRNP F complex with Fox2 to silence fibroblast growth factor receptor 2 exon IIIc. Mauger DM, Lin C, Garcia-Blanco MA. Mol Cell Biol. 2008 Sep . 28(17):5403-19. 10.1128/MCB.00739-08 PubMed 18573884