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p3x-FLAG-hnRNPH1
(Plasmid #21925)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21925 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p3xFLAG-CMV7.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    hnRNP H1
  • Alt name
    hnRNP H
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1353
  • GenBank ID
    NP_005511
  • Entrez Gene
    HNRNPH1 (a.k.a. HNRPH, HNRPH1, hnRNPH)
  • Tag / Fusion Protein
    • 3 Flags (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Note that the 3' end of this insert is extremely difficult to sequence for unknown reasons.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3x-FLAG-hnRNPH1 was a gift from Mariano Garcia-Blanco (Addgene plasmid # 21925 ; http://n2t.net/addgene:21925 ; RRID:Addgene_21925)
  • For your References section:

    hnRNP H and hnRNP F complex with Fox2 to silence fibroblast growth factor receptor 2 exon IIIc. Mauger DM, Lin C, Garcia-Blanco MA. Mol Cell Biol. 2008 Sep . 28(17):5403-19. 10.1128/MCB.00739-08 PubMed 18573884