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pET-Cre
(Plasmid #219798)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219798 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET-19b
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Codon-optimized Cre Recombinase
  • Species
    Phage P1
  • GenBank ID
  • Promoter IPTG-inducible
  • Tag / Fusion Protein
    • HIS-tag for purification

Cloning Information

  • Cloning method Gene Synthesis
  • 5′ sequencing primer TAATACGACTCACTATAGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.05.17.594525 for the bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-Cre was a gift from Gaël Yvert (Addgene plasmid # 219798 ; http://n2t.net/addgene:219798 ; RRID:Addgene_219798)

  • For your References section:

    Kinetic properties of optogenetic site-specific DNA recombination by LiCre-loxP. Dufour A, Duplus-Bottin H, Boukeke-Lesplulier T, Casassa E, Triqueneaux G, Tarbouriech L, Darthenay-Kiennemann C, Dumont A, Moali C, Vittoz F, Jost D, Yvert G. Biol Open. 2026 May 15;15(5):bio062381. doi: 10.1242/bio.062381. Epub 2026 May 14. 10.1242/bio.062381 PubMed 42131951
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