nCas9-NG_P2A-mCherry
(Plasmid
#219934)
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PurposeMammalian expression of Sp-nCas9-NG with P2A-mCherry marker for use with MOBE systems
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Depositing Lab
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Depositing OrganizationUniversity of California, San Diego (UCSD)
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 219934 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 219934-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 219934-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSp-nCas9(D10A)-NG_P2A-mCherry
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SpeciesH. sapiens (human), Synthetic
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MutationnCas9(D10A)
- Promoter CMV
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Tags
/ Fusion Proteins
- SV40 bpNLS (N terminal on insert)
- SV40 bpNLS-P2A-mCherry (C terminal on insert)
Cloning Information
- Cloning method Other
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
QTC24; could also be used for dual-nicking or other editing modalities
DNA (Catalog # 219934-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 219934-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
nCas9-NG_P2A-mCherry was a gift from Alexis Komor (Addgene plasmid # 219934 ; http://n2t.net/addgene:219934 ; RRID:Addgene_219934) -
For your References section:
Development of multiplexed orthogonal base editor (MOBE) systems. Cowan QT, Gu S, Gu W, Ranzau BL, Simonson TS, Komor AC. Nat Biotechnol. 2024 May 21. doi: 10.1038/s41587-024-02240-0. 10.1038/s41587-024-02240-0 PubMed 38773305
