pRSET-MagLOV
(Plasmid
#219957)
-
PurposeBacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 219957 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepRSETB
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2828
- Total vector size (bp) 3263
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameMagLOV
-
SpeciesA. sativa
-
Insert Size (bp)435
-
MutationAsLOV2 C450P L496V Q513K G528K D540M
- Promoter T7
-
Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Described in the article: "Magnetic control of the brightness of fluorescent proteins" https://doi.org/10.5281/zenodo.8137174, https://andrewgyork.github.io/gfp_magnetofluorescence/#MagLOV_update.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pRSET-MagLOV was a gift from Andrew York (Addgene plasmid # 219957 ; http://n2t.net/addgene:219957 ; RRID:Addgene_219957) -
For your References section:
Magnetic control of the brightness of fluorescent proteins. Frank Hayward R, Rai A, Lazzari-Dean, JR, Lefebvre AEYT, York AG, Ingaramo M. (2023) doi: 10.5281/zenodo.8137174 10.5281/zenodo.8137174