mCherry-C1-ORP5deltaTMD-FKBP
(Plasmid
#220073)
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Purposeexpresses ORP5 with the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.
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Depositing Lab
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Depositing OrganizationUniversity of Pittsburgh (University, School of Medicine, and Cancer Institute)
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 220073 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 220073-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 220073-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepmCherry-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOSBPL5deltaTMD
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Alt nameORP5deltaTMD
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SpeciesH. sapiens (human)
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MutationdeltaTMD
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Entrez GeneOSBPL5 (a.k.a. OBPH1, ORP5)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 220073-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 220073-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCherry-C1-ORP5deltaTMD-FKBP was a gift from Gerry Hammond (Addgene plasmid # 220073 ; http://n2t.net/addgene:220073 ; RRID:Addgene_220073) -
For your References section:
Orthogonal Targeting of SAC1 to Mitochondria Implicates ORP2 as a Major Player in PM PI4P Turnover. Doyle CP, Rectenwald A, Timple L, Hammond GRV. Contact (Thousand Oaks). 2024 Feb 7;7:25152564241229272. doi: 10.1177/25152564241229272. eCollection 2024 Jan-Dec. 10.1177/25152564241229272 PubMed 38327560
