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pmiRFP670-C1-Nir1-LNS2
(Plasmid #220085)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220085 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 220085-D50 50 μg of DNA in Tris buffer $495
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DNA 220085-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pmiRFP670-C1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PITPNM3
  • Alt name
    Nir1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    855
  • Mutation
    amino acids 613-897
  • Entrez Gene
    PITPNM3 (a.k.a. ACKR6, CORD5, NIR1, RDGBA3)
  • Promoter CMV
  • Tag / Fusion Protein
    • iRFP670-GGSGGM (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Nir1 sequence corresponds to a rare human allele (17-6455609-G-A (GRCh38) in the gnomAD database) with an A885V missense mutation relative to the reference genome. There is no known phenotype associated with mutation of this residue, and it is not predicted to disrupt membrane interactions. The residue is also not conserved between Nir orthologues.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmiRFP670-C1-Nir1-LNS2 was a gift from Gerry Hammond (Addgene plasmid # 220085 ; http://n2t.net/addgene:220085 ; RRID:Addgene_220085)

  • For your References section:

    PILS-Nir1 is a sensitive phosphatidic acid biosensor that reveals mechanisms of lipid production. Weckerly CC, Rahn TA, Ehrlich M, Wills RC, Pemberton JG, Airola MV, Hammond GRV. J Cell Biol. 2025 Nov 3;224(11):e202405174. doi: 10.1083/jcb.202405174. Epub 2025 Sep 9. 10.1083/jcb.202405174 PubMed 40923975
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