pCIG-LRRC37Bchimpanzee-HA
(Plasmid
#220794)
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PurposeTo express the chimpanzee receptor LRRC37B in mammalian cells together with EGFP
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Depositing Lab
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Depositing OrganizationVlaams Instituut voor Biotechnologie (VIB, vzw)
Located in Belgium -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 220794 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 220794-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 220794-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCIG
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLRRC37B
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SpeciesPan troglodytes (chimpanzee)
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe cDNA was PCR amplified and inserted in the pCIG backbone from a custom made plasmid (Genscript).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 220794-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 220794-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCIG-LRRC37Bchimpanzee-HA was a gift from Pierre Vanderhaeghen (Addgene plasmid # 220794 ; http://n2t.net/addgene:220794 ; RRID:Addgene_220794) -
For your References section:
LRRC37B is a human modifier of voltage-gated sodium channels and axon excitability in cortical neurons. Libe-Philippot B, Lejeune A, Wierda K, Louros N, Erkol E, Vlaeminck I, Beckers S, Gaspariunaite V, Bilheu A, Konstantoulea K, Nyitrai H, De Vleeschouwer M, Vennekens KM, Vidal N, Bird TW, Soto DC, Jaspers T, Dewilde M, Dennis MY, Rousseau F, Comoletti D, Schymkowitz J, Theys T, de Wit J, Vanderhaeghen P. Cell. 2023 Dec 21;186(26):5766-5783.e25. doi: 10.1016/j.cell.2023.11.028. 10.1016/j.cell.2023.11.028 PubMed 38134874