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(Plasmid #22082)


Item Catalog # Description Quantity Price (USD)
Plasmid 22082 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5448
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    DH5 alpha or XL1 @ 37˚C
  • Copy number
    High Copy


  • Gene/Insert name
    von Hippel-Lindau tumor suppressor (VHL)
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    point mutant converting aa 167 from Arginine to Tryptophane (R167W)
  • GenBank ID
  • Entrez Gene
    VHL (a.k.a. HRCA1, RCA1, VHL1, pVHL)
  • Tag / Fusion Protein
    • HA-tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (destroyed during cloning)
  • 3′ cloning site Xba I (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-VHL R167W-pRc/CMV was a gift from William Kaelin (Addgene plasmid # 22082 ; ; RRID:Addgene_22082)
  • For your References section:

    Binding of the von Hippel-Lindau tumor suppressor protein to Elongin B and C. Kibel A, Iliopoulos O, DeCaprio JA, Kaelin WG. Science. 1995 Sep 8. 269(5229):1444-6. 10.1126/science.7660130 PubMed 7660130