HA-VHL R167W-pRc/CMV
(Plasmid #22082)

• Depositing Lab: William Kaelin
• Publication: Kibel et al Science. 1995 Sep 8. 269(5229):1444-6.

Backbone

• Vector backbone: pRc/CMV
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5448
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5 alpha or XL1 @ 37˚C
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: von Hippel-Lindau tumor suppressor (VHL)
• Alt name: vHL
• Species: H. sapiens (human)
• Insert Size (bp): 745
• Mutation: point mutant converting aa 167 from Arginine to Tryptophane (R167W)
• GenBank ID: NM_000551
• Entrez Gene: VHL (a.k.a. HRCA1, RCA1, VHL1, pVHL)
• Tag / Fusion Protein:
  • HA-tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (destroyed during cloning)
• 3′ cloning site: Xba I (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry