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EF1a-dCasRx-RBM25
(Plasmid #221001)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221001 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 221001-D50 50 μg of DNA in Tris buffer $495
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DNA 221001-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pXR002 (Addgene #109050)
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCasRx-RBM25
  • Alt name
    Human RBM25 fused C-terminal of dCasRx
  • Species
    H. sapiens (human)
  • Promoter EF1a
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS-HA (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Backbone and dCasRx coding sequence from Konermann et al., 2018 (Addgene #109050)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Co-transfect with gRNA vector at a 1:1 ratio. Highly efficient transfection is required for optimal transient splicing modulation. For hard to transfect cells we recommend either sorting for BFP expression (on the gRNA vector), or using the stable expression system. For additional details, please see our paper.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EF1a-dCasRx-RBM25 was a gift from Benjamin Blencowe & Mikko Taipale (Addgene plasmid # 221001 ; http://n2t.net/addgene:221001 ; RRID:Addgene_221001)

  • For your References section:

    Efficient, specific, and combinatorial control of endogenous exon splicing with dCasRx-RBM25. Li JD, Taipale M, Blencowe BJ. Mol Cell. 2024 Jul 11;84(13):2573-2589.e5. doi: 10.1016/j.molcel.2024.05.028. Epub 2024 Jun 24. 10.1016/j.molcel.2024.05.028 PubMed 38917795
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