|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22132||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7394
Vector typeMammalian Expression, Retroviral, RNAi
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsDH5a or HB101
Copy numberHigh Copy
Gene/Insert namesynthethic oligonucleotide
Insert Size (bp)58
Mutationsynthethic oligonucleotide encoding shRNA targeting human HIF2alpha mRNA
- Cloning method Restriction Enzyme
- 5′ cloning site Bgl II (destroyed during cloning)
- 3′ cloning site Hind III (not destroyed)
- 5′ sequencing primer MSCV reverse (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
shRNA oligo sequence - 5'-GAGATCCCCGGAGACGGAGGTGTTCTATTTCAAGAGAATAGAACACCTCCGTCTCCTTTT
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:shHIF2α(#2) (HIF2α-#3-pSUPER-retro-GFP/Neo) was a gift from William Kaelin (Addgene plasmid # 22132 ; http://n2t.net/addgene:22132 ; RRID:Addgene_22132)
For your References section:Hypoxia-inducible factor linked to differential kidney cancer risk seen with type 2A and type 2B VHL mutations. Li L, Zhang L, Zhang X, Yan Q, Minamishima YA, Olumi AF, Mao M, Bartz S, Kaelin WG. Mol Cell Biol. 2007 Aug . 27(15):5381-92. 10.1128/MCB.00282-07 PubMed 17526729