FHpHG_mTent5a
(Plasmid
#221439)
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PurposeTransfection into the cells for the reporter or qPCR assay.
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Depositing Lab
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Depositing OrganizationNational Institute of Environmental Health Sciences (NIEHS)
Located in the United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 221439 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 221439-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 221439-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCDH_CMV
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTent5a
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SpeciesM. musculus (mouse)
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Entrez GeneFam46a (a.k.a. D930050G01Rik)
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Tag
/ Fusion Protein
- FLAG-2xHA-(HRV 3C site)-6xHis-EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsiWI (unknown if destroyed)
- 3′ cloning site SacII (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 221439-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 221439-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FHpHG_mTent5a was a gift from Marcos Morgan (Addgene plasmid # 221439 ; http://n2t.net/addgene:221439 ; RRID:Addgene_221439) -
For your References section:
TENT5C functions as a corepressor in the ligand-bound glucocorticoid receptor and estrogen receptor alpha complexes. Li Y, Perera L, He RS, Baptissart M, Petrovich RM, Morgan M. FEBS J. 2025 May 27. doi: 10.1111/febs.70137. 10.1111/febs.70137 PubMed 40421654
Map uploaded by the depositor.
