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PurposeExpress OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.
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Depositing Lab
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Depositing OrganizationProfluent Bio
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 221566 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 * | |
| DNA | 221566-D50 | 50 μg of DNA in Tris buffer | $495 * | ||
| DNA | 221566-D100 | 100 μg of DNA in Tris buffer | $585 * | ||
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Backbone
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Vector backbonepTwist CMV
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Backbone manufacturerTwist
- Backbone size w/o insert (bp) 4303
- Total vector size (bp) 5101
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Modifications to backboneRemoved repeated rrnG and T7 terminators to increase plasmid stability. Added GFP reporter cassette.
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOpenCRISPR-1 sgRNA 16nt stem loop
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Alt nameOpenCRISPR1 sgRNA 16nt stem loop
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SpeciesH. sapiens (human)
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Insert Size (bp)1796
- Promoter U6
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Whole plasmid sequencing
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.04.22.590591 for bioRxiv preprint.
DNA (Catalog # 221566-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 221566-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
OpenCRISPR-1 sgRNA-16nt stem was a gift from Ali Madani (Addgene plasmid # 221566 ; http://n2t.net/addgene:221566 ; RRID:Addgene_221566) -
For your References section:
Design of highly functional genome editors by modelling CRISPR-Cas sequences. Ruffolo JA, Nayfach S, Gallagher J, Bhatnagar A, Beazer J, Hussain R, Russ J, Yip J, Hill E, Pacesa M, Meeske AJ, Cameron P, Madani A. Nature. 2025 Jul 30. doi: 10.1038/s41586-025-09298-z. 10.1038/s41586-025-09298-z PubMed 40739342
Map uploaded by the depositor.
