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LAMP1-Halo
(Plasmid #221655)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221655 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 221655-D50 50 μg of DNA in Tris buffer $495
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DNA 221655-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pHTC
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 4994
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LAMP1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1200
  • GenBank ID
    25328
  • Entrez Gene
    Lamp1 (a.k.a. LGP120)
  • Promoter CMV
  • Tag / Fusion Protein
    • HaloTag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (destroyed during cloning)
  • 3′ cloning site Nit1 (not destroyed)
  • 5′ sequencing primer 5'-GGATTTGCCCATACCGATCAGG
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

LAMP1 insert recloned from Addgene 1817

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LAMP1-Halo was a gift from Erika Holzbaur (Addgene plasmid # 221655 ; http://n2t.net/addgene:221655 ; RRID:Addgene_221655)

  • For your References section:

    Mitochondrial damage triggers the concerted degradation of negative regulators of neuronal autophagy. Basak B, Holzbaur ELF. Nat Commun. 2025 Aug 9;16(1):7367. doi: 10.1038/s41467-025-62379-5. 10.1038/s41467-025-62379-5 PubMed 40783388
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