|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22226||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ cloning site BstXI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer EF-1a Forward (Common Sequencing Primers)
This vector was the backbone for several of the Ananda Roy plasmids.
SpeI and KpnI site are missing from the MCS, unlike the original Baltimore vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEBB modified was a gift from David Baltimore (Addgene plasmid # 22226 ; http://n2t.net/addgene:22226 ; RRID:Addgene_22226)
For your References section:Differential inhibition of signaling pathways by dominant-negative SH2/SH3 adapter proteins. Tanaka M, Gupta R, Mayer BJ. Mol Cell Biol. 1995 Dec . 15(12):6829-37. PubMed 8524249