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peCALWY-NB
(Plasmid #22239)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22239 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 22239-D50 50 μg of DNA in Tris buffer $495
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DNA 22239-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCMV (Clontech)
  • Backbone size w/o insert (bp) 3900
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    E.coli nova blue LB culture medium 30 ug/ml kanamycin Culture overnight, 37 C/250 rpm
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eCALWY non-binding variant
  • Alt name
    eCALWY-NB
  • Species
    H. sapiens (human); GFP's
  • Insert Size (bp)
    2100
  • Mutation
    C284S, C287S, C413S and C416S mutations linker consists of 9 GGSGGS repeats

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV promotor
  • 3′ sequencing primer CMV terminator
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    peCALWY-NB was a gift from Maarten Merkx (Addgene plasmid # 22239 ; http://n2t.net/addgene:22239 ; RRID:Addgene_22239)

  • For your References section:

    Genetically encoded FRET sensors to monitor intracellular Zn(2+) homeostasis. Vinkenborg JL, Nicolson TJ, Bellomo EA, Koay MS, Rutter GA, Merkx M. Nat Methods. 2009 Oct;6(10):737-40. doi: 10.1038/nmeth.1368. 10.1038/nmeth.1368 PubMed 19718032
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