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pSR2001
(Plasmid #222626)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222626 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pAN1717
  • Backbone size (bp) 8971
  • Modifications to backbone
    Replaced eYFP cassette with Sp-dCas9 gene and LacZa cassette (including PTac and Sp-dCas9 sgRNA scaffold) with BsaI recognition sites for insertion of gRNA spacer sequence
  • Vector type
    Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSR2001 was a gift from Lauren Andrews (Addgene plasmid # 222626 ; http://n2t.net/addgene:222626 ; RRID:Addgene_222626)
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