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pSR3034
(Plasmid #222628)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222628 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pSR2001
  • Backbone size w/o insert (bp) 8971
  • Total vector size (bp) 8616
  • Modifications to backbone
    Replaced LacZa cassette with gRNA spacer sequence targeting lapC
  • Vector type
    Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    gRNA spacer sequence targeting lapC
  • gRNA/shRNA sequence
    agtgcccccaactgaccatc
  • Species
    Synthetic
  • Promoter PTac

Cloning Information

  • Cloning method Golden Gate
  • 5′ sequencing primer cgacgtgtataccttct
  • 3′ sequencing primer cggtctgataagagaca
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSR3034 was a gift from Lauren Andrews (Addgene plasmid # 222628 ; http://n2t.net/addgene:222628 ; RRID:Addgene_222628)
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