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OgeuIscB CTG RNA
(Plasmid #222865)

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Item Catalog # Description Quantity Price (USD)
Plasmid 222865 Standard format: Plasmid sent in bacteria as agar stab 1 $94 *
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DNA 222865-D50 50 μg of DNA in Tris buffer $495 *
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DNA 222865-D100 100 μg of DNA in Tris buffer $585 *
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OgeuIscB omega RNA with a (CTG)6 target sequence
  • gRNA/shRNA sequence
    GCTGCTGCTGCTGCTGCTG
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    225
  • Promoter Human U6

Cloning Information

  • Cloning method Unknown

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The original plasmid is pHS0812 (Addgene #176541). The plasmid was digested with BbsI enzyme and a sticky-end ligation was performed to add a 19bp target sequence.

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.10.24.563270 for bioRxiv preprint.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    OgeuIscB CTG RNA was a gift from Vincent Dion (Addgene plasmid # 222865 ; http://n2t.net/addgene:222865 ; RRID:Addgene_222865)

  • For your References section:

    Developing small Cas9 hybrids using molecular modeling. Mangin A, Dion V, Menzies G. bioRxiv 2023.10.24.563270 10.1101/2023.10.24.563270
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