|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22290||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6112
Vector typeYeast Expression
Growth in Bacteria
Alt namerad5-ATPase mutant
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)3510
Entrez GeneRAD5 (a.k.a. YLR032W, REV2, SNM2)
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13_pUC-rev
- 3′ sequencing primer M13_puc-for (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Mutation inactivates the ATPase and DNA helicase activities of RAD5
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pR5-30 was a gift from Louise Prakash (Addgene plasmid # 22290 ; http://n2t.net/addgene:22290 ; RRID:Addgene_22290)
For your References section:Requirement of Rad5 for DNA polymerase zeta-dependent translesion synthesis in Saccharomyces cerevisiae. Pages V, Bresson A, Acharya N, Prakash S, Fuchs RP, Prakash L. Genetics. 2008 Sep . 180(1):73-82. 10.1534/genetics.108.091066 PubMed 18757916