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pEGFPC1-FLAG-Synaptojanin 1-170
(Plasmid #22294)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22294 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 22294-D50 50 μg of DNA in Tris buffer $495
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DNA 22294-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Synaptojanin 1_170
  • Alt name
    inositol 5'-phosphatase p170 isoform
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4500
  • GenBank ID
    AF009040
  • Entrez Gene
    SYNJ1 (a.k.a. DEE53, EIEE53, INPP5G, PARK20)
  • Tags / Fusion Proteins
    • GFP (N terminal on backbone)
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer EGFP-N Sequencing Primer
  • 3′ sequencing primer EGFP-C Sequencing Primer
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFPC1-FLAG-Synaptojanin 1-170 was a gift from Pietro De Camilli (Addgene plasmid # 22294 ; http://n2t.net/addgene:22294 ; RRID:Addgene_22294)

  • For your References section:

    Two synaptojanin 1 isoforms are recruited to clathrin-coated pits at different stages. Perera RM, Zoncu R, Lucast L, De Camilli P, Toomre D. Proc Natl Acad Sci U S A. 2006 Dec 19. 103(51):19332-7. 10.1073/pnas.0609795104 PubMed 17158794
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