pGBScrispri Sham
(Plasmid
#223201)
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PurposeGroup B Streptococcus inducible dCas12 CRISPRi with Modular crRNA
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Depositing Lab
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Depositing OrganizationUniversity of Pittsburgh (University, School of Medicine, and Cancer Institute)
Located in the United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 223201 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 223201-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 223201-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepJC005.em
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Erythromycin, 200 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameN/A
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 223201-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 223201-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGBScrispri Sham was a gift from Thomas Hooven (Addgene plasmid # 223201 ; http://n2t.net/addgene:223201 ; RRID:Addgene_223201) -
For your References section:
A Cas12a Toolbox for Rapid and Flexible Group B Streptococcus Genomic Editing and CRISPRi. Hillebrand GH, Carlin SC, Giacobe EJ, Stephenson HA, Collins J, Hooven TA. Mol Microbiol. 2025 Nov;124(5):449-461. doi: 10.1111/mmi.70022. Epub 2025 Sep 13. 10.1111/mmi.70022 PubMed 40944362
