YFP-CAAX
(Plasmid
#223558)
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PurposePlasmid expressing YFP(Venus) followed by the last 25 amino acids of human KRas4b including the polybasic regions and CAAX prenylation box
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Depositing Lab
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Depositing OrganizationBoston University Medical Campus
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 223558 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 * | |
| DNA | 223558-D50 | 50 μg of DNA in Tris buffer | $495 * | ||
| DNA | 223558-D100 | 100 μg of DNA in Tris buffer | $585 * | ||
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Backbone
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Vector backbonepVenus-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameYFP(Venus) - KRas4b
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Alt nameVenus-kras
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SpeciesSynthetic
Resource Information
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A portion of this plasmid was derived from a plasmid made byReceived from Nevin Lambert, Augusta University, Augusta, GA.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 223558-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 223558-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YFP-CAAX was a gift from Mikel Garcia-Marcos (Addgene plasmid # 223558 ; http://n2t.net/addgene:223558 ; RRID:Addgene_223558) -
For your References section:
A sensitive biosensor of endogenous Galpha(i) activity enables the accurate characterization of endogenous GPCR agonist responses. Luebbers A, Janicot R, Zhao J, Philibert CE, Garcia-Marcos M. Sci Signal. 2025 Mar 25;18(879):eadp6457. doi: 10.1126/scisignal.adp6457. Epub 2025 Mar 25. 10.1126/scisignal.adp6457 PubMed 40132053
