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DIO-shRNA(VGluT2)
(Plasmid #223683)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223683 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    unknown
  • Vector type
    AAV, RNAi, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    100 ug/ml
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DIO-(EGFP-U6)-VGluT2 shRNA
  • gRNA/shRNA sequence
    5’-GGCAAAGTTATCAAGGAGAAA-3’
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Slc17a6 (a.k.a. 2900073D12Rik, DNPI, VGLUT2)
  • Tag / Fusion Protein
    • GFP

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    BrainVTA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DIO-shRNA(VGluT2) was a gift from David H Root (Addgene plasmid # 223683 ; http://n2t.net/addgene:223683 ; RRID:Addgene_223683)

  • For your References section:

    Distinct properties of glutamate-dopamine, nonglutamate-dopamine, and glutamate-only ventral tegmental area neurons. Prevost ED, Ward LA, Alas D, A Aimale G, K Ikenberry S, Fox K, Deming M, Pelletier J, Ly A, Ball J, Kilpatrick ZP, Price K, Polter AM, Simoes de Souza F, Fulgham S, Kim YS, Fenno LE, Ramakrishnan C, Deisseroth K, Root DH. Sci Signal. 2026 Aug 18;19(951):eaee2028. doi: 10.1126/scisignal.aee2028. Epub 2026 Aug 18. 10.1126/scisignal.aee2028 PubMed 42611973
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