DIO-shRNA(TH)
(Plasmid
#223686)
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PurposeExpresses EGFP and TH shRNA in Cre-defined neurons
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Depositing Lab
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Depositing OrganizationUniversity of Colorado, Boulder
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 223686 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneunknown
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Vector typeAAV, RNAi, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructions100 ug/ml
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDIO-(EGFP-U6)-TH shRNA
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SpeciesM. musculus (mouse)
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Entrez GeneTh
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Tag
/ Fusion Protein
- GFP
Resource Information
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A portion of this plasmid was derived from a plasmid made byBrainVTA
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DIO-shRNA(TH) was a gift from David H Root (Addgene plasmid # 223686 ; http://n2t.net/addgene:223686 ; RRID:Addgene_223686) -
For your References section:
Distinct properties of glutamate-dopamine, nonglutamate-dopamine, and glutamate-only ventral tegmental area neurons. Prevost ED, Ward LA, Alas D, A Aimale G, K Ikenberry S, Fox K, Deming M, Pelletier J, Ly A, Ball J, Kilpatrick ZP, Price K, Polter AM, Simoes de Souza F, Fulgham S, Kim YS, Fenno LE, Ramakrishnan C, Deisseroth K, Root DH. Sci Signal. 2026 Aug 18;19(951):eaee2028. doi: 10.1126/scisignal.aee2028. Epub 2026 Aug 18. 10.1126/scisignal.aee2028 PubMed 42611973