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pAc5.1B-lambdaN-HA-DmGW182
(Plasmid #22406)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22406 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAc5.1B-lambdaN
  • Backbone manufacturer
    Elisa Izaurralde
  • Backbone size w/o insert (bp) 5459
  • Vector type
    Drosophila expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Dh5 alpha and XL1-blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gawky, variant A
  • Alt name
    GW182
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    4155
  • GenBank ID
    NM_166780
  • Entrez Gene
    gw (a.k.a. Dmel_CG31992, CG11484, CG31992, CG9905, Dmel\CG31992, GQ182, GW, GW182, Gw, Gw182, anon-EST:Posey205, dGW182, gw182)
  • Tag / Fusion Protein
    • lambdaN-HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer Ac5 forward primer site
  • 3′ sequencing primer BGH reverse priming site
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc5.1B-lambdaN-HA-DmGW182 was a gift from Elisa Izaurralde (Addgene plasmid # 22406 ; http://n2t.net/addgene:22406 ; RRID:Addgene_22406)
  • For your References section:

    mRNA degradation by miRNAs and GW182 requires both CCR4:NOT deadenylase and DCP1:DCP2 decapping complexes. Behm-Ansmant I, Rehwinkel J, Doerks T, Stark A, Bork P, Izaurralde E. Genes Dev. 2006 Jul 15. 20(14):1885-98. 10.1101/gad.1424106 PubMed 16815998