pEZYeGFP-SARS-CoV-2E GGG
(Plasmid
#224582)
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PurposeTo express a GFP tagged version of the Envelope protein of SARS-CoV-2 with a mutated PBM in mammalian cells
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Depositing Lab
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Depositing OrganizationCentro Nacional de Biotecnologia (CNB)
Located in Spain -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 224582 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 224582-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 224582-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEZYeGFP
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Backbone manufacturerpEGFPC4 converted by Yu-Zhu Zhan lab. Addgene 18671
- Backbone size w/o insert (bp) 7040
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSARS-CoV-2E GGG
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SpeciesSARS-CoV-2
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Insert Size (bp)228
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MutationPBM (LLV) mutated to GGG
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Entrez GeneE (a.k.a. GU280_gp04)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer EGFP-C
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 224582-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 224582-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEZYeGFP-SARS-CoV-2E GGG was a gift from Isabel Mérida (Addgene plasmid # 224582 ; http://n2t.net/addgene:224582 ; RRID:Addgene_224582) -
For your References section:
Identification of Host PDZ-Based Interactions with the SARS-CoV-2 E Protein in Human Monocytes. Avila-Flores A, Sanchez-Cabezon JJ, Ochoa-Echeverria A, Checa AI, Rosas-Garcia J, Tellez-Araiza M, Casado S, Liebana R, Santos-Mendoza T, Merida I. Int J Mol Sci. 2023 Aug 14;24(16):12793. doi: 10.3390/ijms241612793. 10.3390/ijms241612793 PubMed 37628973
