|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22462||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typeEntry Vector
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Insert Size (bp)700
Entrez GeneeGFP (a.k.a. pPRS3a_01)
- Cloning method Gateway Cloning
- 5′ sequencing primer M13_puc_F
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This clone was amplified with an attB2 site, at the 5’ end and a attB3 site on the 3’ end. The product was then recombined with the pDNR P2R-P3 vector.
Can be used to make 3’ end (C-Term) tagged fusion construct.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p3Eegfp was a gift from Nathan Lawson (Addgene plasmid # 22462 ; http://n2t.net/addgene:22462 ; RRID:Addgene_22462)
For your References section:Gateway compatible vectors for analysis of gene function in the zebrafish. Villefranc JA, Amigo J, Lawson ND. Dev Dyn. 2007 Nov . 236(11):3077-87. 10.1002/dvdy.21354 PubMed 17948311