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ph2p
(Plasmid #22520)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22520 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin(Zeocin)
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MPyV VP2
  • Alt name
    Mouse Polyomavirus
  • Alt name
    Murine Polyomavirus
  • Alt name
    codon modified
  • Species
    Murine Polyomavirus
  • Insert Size (bp)
    959
  • GenBank ID
    FJ548571

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attR1 (destroyed during cloning)
  • 3′ cloning site attR2 (destroyed during cloning)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

First reference to this plasmid was in:

Human Merkel cell polyomavirus infection II. MCV is a common human infection that can be detected by conformational capsid epitope immunoassays. Tolstov YL, Pastrana DV, Feng H, Becker JC, Jenkins FJ, Moschos S, Chang Y, Buck CB, Moore PS. Int J Cancer. 2009 Sep 15.125(6):1250-6

Genbank style annotations can be found at:
http://home.ccr.cancer.gov/LCO/packaging.htm

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ph2p was a gift from Christopher Buck (Addgene plasmid # 22520)
  • For your References section:

    Quantitation of human seroresponsiveness to Merkel cell polyomavirus. Pastrana DV, Tolstov YL, Becker JC, Moore PS, Chang Y, Buck CB. PLoS Pathog. 2009 Sep . 5(9):e1000578. 10.1371/journal.ppat.1000578 PubMed 19750217