Kif1bβ-pcDNA3
(Plasmid #22534)

• Depositing Lab: William Kaelin
• Publication: Schlisio et al Genes Dev. 2008 Apr 1. 22(7):884-93.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5362
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha, XL1
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kif1bβ
• Alt name: kinesin family member 1B (KIF1B), transcript variant 1 (beta)
• Species: H. sapiens (human)
• Insert Size (bp): 5313
• Mutation: wt cDNA
• GenBank ID: NM_015074
• Entrez Gene: KIF1B (a.k.a. CMT2, CMT2A, CMT2A1, HMSNII, KLP, NBLST1)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn I (not destroyed)
• 3′ cloning site: Xba I (not destroyed)
• 5′ sequencing primer: T7 or CMV-forward
• 3′ sequencing primer: SP6 or BGH-reverse

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry