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aTY48_pPGK1_Kcad_IRES_PuroR_WPRE
(Plasmid #225347)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225347 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pHR
  • Backbone manufacturer
    pHR backbone Addgene plasmid 60906
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kcad
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Cdh6 (a.k.a. cad6)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pHR backbone Addgene plasmid 60906 (Lentiviral backbone pAN2424 - Gift from Andrew Ng from El Samad Lab UCSF – pHR backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    aTY48_pPGK1_Kcad_IRES_PuroR_WPRE was a gift from Wendell Lim (Addgene plasmid # 225347 ; http://n2t.net/addgene:225347 ; RRID:Addgene_225347)

  • For your References section:

    Synthetic organizer cells guide development via spatial and biochemical instructions. Yamada T, Trentesaux C, Brunger JM, Xiao Y, Stevens AJ, Martyn I, Kasparek P, Shroff NP, Aguilar A, Bruneau BG, Boffelli D, Klein OD, Lim WA. Cell. 2024 Dec 11:S0092-8674(24)01323-0. doi: 10.1016/j.cell.2024.11.017. 10.1016/j.cell.2024.11.017 PubMed 39706189
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