pUb-G76V-GFP-IRES-DsRed
(Plasmid #225503)

• Purpose: Expression of Ub-G76V-GFP along dsRED from one mRNA
• Depositing Lab: David Rubinsztein
• Depositing Organization: University of Cambridge
  Located in the United Kingdom of Great Britain and Northern Ireland
• Publication: Yu et al Hum Mol Genet. 2024 May 22:ddae085. doi: 10.1093/hmg/ddae085.

Backbone

• Vector backbone: pIRES2 DsRed-Express2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5262
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418) ; DsRed

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Ub-G76V-GFP
• Alt name: Ubiquitin
• Alt name: Ub
• Species: H. sapiens (human)
• Insert Size (bp): 990
• Mutation: Ubiquitin fused to N-terminus of GFP. Glycine 76 mutated to Valine.
• Promoter: CMV promoter
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Nico Dantuma, Addgene plasmid #11941

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was created by Dr Lidia Wrobel in the Rubinsztein lab.

DNA (Catalog # 225503-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 225503-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.