pATB_142
(Plasmid #226033)

• Purpose: Plasmid encodes an AT-FP with OprF-spacer signal peptide, flex linker, EhaA translocation unit enabling the display of a passenger of interest at the bacterial cell surface
• Depositing Lab: Joachim Jose
• Publication: Furtmann et al Commun Biol. 2026 May 21;9(1):694. doi: 10.1038/s42003-026-10324-7.

Backbone

• Vector backbone: pBBR1-MCS2 (Kovach et al., 1995)
• Backbone size w/o insert (bp): 7509
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: default passenger (=insert) is cel5
• Alt name: UniProt Q2SFD8
• Species: Hahella chejuensis
• Insert Size (bp): 1729
• Promoter: Arabinose inducible (araBAD)
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

AT-FP = Autotransporter fusion protein