pAAV_Ckm_F9
(Plasmid #226140)

• Purpose: AAV construct for HITI insert with Ckm gRNA and hF9 transgene
• Depositing Lab: Christopher Nelson
• Publication: Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4):102320. doi: 10.1016/j.omtn.2024.102320. eCollection 2024 Dec 10.

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Backbone

• Vector backbone: pAAV
• Total vector size (bp): 7192
• Vector type: Mammalian Expression, AAV, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: F9
• Species: H. sapiens (human)
• Insert Size (bp): 2774
• Entrez Gene: F9 (a.k.a. F9 p22, FIX, HEMB, P19, PTC, THPH8)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: hU6 promoter

Gene/Insert 2

• Gene/Insert name: Ckm gRNA
• Alt name: Ckm
• Species: M. musculus (mouse)
• Entrez Gene: Ckm (a.k.a. CPK-M, Ckmm, M-CK, MCK)

Gene/Insert 3

• Gene/Insert name: EGFP

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Please visit https://doi.org/10.1101/2024.03.18.582796 for bioRxiv preprint.