Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pEF1a-mRor2Y641,645,646F
(Plasmid #22638)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22638 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEF1a-myc/his A
  • Backbone size w/o insert (bp) 9226
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ror2 Y641,645,646F
  • Alt name
    Ror2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2835
  • Mutation
    Changed Tyrosine 641, 645 and 646 to Phenylalanine
  • GenBank ID
    AB010384
  • Entrez Gene
    Ror2 (a.k.a. Ntr, Ntrkr2)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Y. Minami (Japan)
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF1a-mRor2Y641,645,646F was a gift from Roel Nusse (Addgene plasmid # 22638 ; http://n2t.net/addgene:22638 ; RRID:Addgene_22638)
  • For your References section:

    The Ror2 receptor requires tyrosine kinase activity to mediate Wnt5a signaling. Mikels A, Minami Y, Nusse R. J Biol Chem. 2009 Aug 31. ():. 10.1074/jbc.M109.041715 PubMed 19720827