pR-Avr
(Plasmid #22650)

• Depositing Lab: Lynn Hudson
• Publication: Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-90.

Backbone

• Vector backbone: pGEM-3Z
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 2743
• Vector type: Cloning and RNA Synthesis
• Selectable markers: LacZ

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Myelin Transcription Factor 1
• Alt name: Myt1
• Species: R. norvegicus (rat)
• Insert Size (bp): 700
• Entrez Gene: Myt1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7 Promoter
• 3′ sequencing primer: SP6 Promoter

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Used to make riboprobes by cutting with SmaI and transcribing with SP6 polymerase to give a 700 bp antisense riboprobe or HindIII and transcribing with T7 polymerase to give a 700bp sense riboprobe for RNA analysis on Northern blots. For 3' UTR of cDNA

This Rat Myt1 DNA cloned by Hudson lab.