pRP.CEP290 c.2991+1655A>G
(Plasmid
#226556)
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PurposeMinigene encoding the CEP290 c.2991+1655A>G mutation
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Depositing Lab
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Depositing OrganizationInstitute of Molecular and Cell Biology, A*STAR Research Entities
Located in Singapore -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 226556 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 226556-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 226556-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepRP
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Backbone manufacturerVectorBuilder
- Backbone size w/o insert (bp) 2297
- Total vector size (bp) 8779
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCEP290
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SpeciesH. sapiens (human)
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Insert Size (bp)6728
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Mutationc.2991+1655A>G intronic mutation
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GenBank ID
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Entrez GeneCEP290 (a.k.a. 3H11Ag, BBS14, CT87, JBTS5, LCA10, MKS4, NPHP6, POC3, SLSN6, rd16)
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Tag
/ Fusion Protein
- 3xFLAG (N terminal on insert)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 226556-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 226556-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRP.CEP290 c.2991+1655A>G was a gift from Dave Wee (Addgene plasmid # 226556 ; http://n2t.net/addgene:226556 ; RRID:Addgene_226556) -
For your References section:
Unveiling sequence-agnostic mixed-chemical modification patterns for splice-switching oligonucleotides using the NATURA platform. Tabaglio T, Agarwal T, Cher WY, Ow JR, Chew AK, Sun PYQ, Reddy Gurrampati RS, Lu H, Naidu P, Ng HK, Le Guezennec X, Ng SY, Lakshmanan M, Guccione E, Wee KB. Mol Ther Nucleic Acids. 2025 Jan 7;36(1):102422. doi: 10.1016/j.omtn.2024.102422. eCollection 2025 Mar 11. 10.1016/j.omtn.2024.102422 PubMed 39926316
Map uploaded by the depositor.
