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mNeonGreen-Sec24D
(Plasmid #226581)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226581 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 226581-D50 50 μg of DNA in Tris buffer $495
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DNA 226581-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    mNeonGreen-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sec24D (Sec24 homolog D)
  • Alt name
    COPII coat complex component
  • Species
    H. sapiens (human)
  • Entrez Gene
    SEC24D (a.k.a. CLCRP2)
  • Tag / Fusion Protein
    • mNeonGreen (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mNeonGreen-Sec24D was a gift from Gia Voeltz (Addgene plasmid # 226581 ; http://n2t.net/addgene:226581 ; RRID:Addgene_226581)

  • For your References section:

    Vesicular and uncoated Rab1-dependent cargo carriers facilitate ER to Golgi transport. Westrate LM, Hoyer MJ, Nash MJ, Voeltz GK. J Cell Sci. 2020 Jul 24;133(14). pii: jcs.239814. doi: 10.1242/jcs.239814. 10.1242/jcs.239814 PubMed 32616562
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