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pTol2Dest_ziwi:loxp-stop-loxp-mCherry_pGH
(Plasmid #226785)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226785 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pTol2Dest-pGH_R4-R3
  • Backbone manufacturer
    Unknown
  • Backbone size w/o insert (bp) 9708
  • Total vector size (bp) 15299
  • Vector type
    Cre/Lox ; Zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    ziwi promoter
  • Alt name
    piwil1
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    4824
  • Entrez Gene
    piwil1 (a.k.a. ziwi)

Gene/Insert 2

  • Gene/Insert name
    loxp-stop-loxp
  • Species
    Synthetic
  • Insert Size (bp)
    1343

Gene/Insert 3

  • Gene/Insert name
    mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    708

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This construct was cloned by Miranda L. Wilson. p5E-ziwi was a gift from Bruce W. Draper. pME-loxp-stop-loxp: Tol2kit 2.0 p3E-mCherry: (Ariotti & Rae et al., 2018), Addgene plasmid #108884 "p3E-mCherry was a gift from Rob Parton (Addgene plasmid # 108884 ; http://n2t.net/addgene:108884 ; RRID:Addgene_108884) "

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTol2Dest_ziwi:loxp-stop-loxp-mCherry_pGH was a gift from Florence Marlow (Addgene plasmid # 226785 ; http://n2t.net/addgene:226785 ; RRID:Addgene_226785)

  • For your References section:

    Primordial germ cells differentially contribute to the germline in zebrafish. Pahima M, Marlow FL. Development. 2026 Jul 6:dev.204904. doi: 10.1242/dev.204904. 10.1242/dev.204904 PubMed 42403048
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