pME-pACre
(Plasmid
#226789)
-
PurposeMiddle-entry vector with photoactivatable Cre recombinase (pACre). pACre activated with 470nm Blue Light. For use in Gateway cloning.
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 226789 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepME-MCS
-
Backbone manufacturerTol2 v2.0
- Backbone size w/o insert (bp) 2765
- Total vector size (bp) 4778
-
Vector typeCre/Lox ; Gateway cloning middle-entry vector
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namepACre (Photoactivatable Cre)
-
Alt namePA-Cre
-
SpeciesSynthetic
-
Insert Size (bp)2043
Resource Information
-
A portion of this plasmid was derived from a plasmid made byThis construct was cloned by Maya Pahima. (Kawano et al., 2016), Addgene plasmid #122960 "pcDNA3.1_PA-Cre was a gift from Moritoshi Sato (Addgene plasmid # 122960 ; http://n2t.net/addgene:122960 ; RRID:Addgene_122960) "
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pME-pACre was a gift from Florence Marlow (Addgene plasmid # 226789 ; http://n2t.net/addgene:226789 ; RRID:Addgene_226789) -
For your References section:
Primordial germ cells differentially contribute to the germline in zebrafish. Pahima M, Marlow FL. Development. 2026 Jul 6:dev.204904. doi: 10.1242/dev.204904. 10.1242/dev.204904 PubMed 42403048