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pCSDest2_pACre-nos3'utr
(Plasmid #226790)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226790 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCSDest2
  • Backbone manufacturer
    Nathan Lawson
  • Backbone size w/o insert (bp) 5800
  • Total vector size (bp) 7105
  • Vector type
    Cre/Lox ; For IVT

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    pACre (Photoactivatable Cre)
  • Alt name
    PA-Cre
  • Species
    Synthetic
  • Insert Size (bp)
    2043

Gene/Insert 2

  • Gene/Insert name
    nanos3, 3'utr
  • Alt name
    nos3, 3'utr
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    599

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This construct was cloned by Maya Pahima. Backbone: (Villefranc et al., 2007), Addgene plasmid #22424 "pCSDest2 was a gift from Nathan Lawson (Addgene plasmid # 22424 ; http://n2t.net/addgene:22424 ; RRID:Addgene_22424)" pACre: (Kawano et al., 2016), Addgene plasmid #122960 "pcDNA3.1_PA-Cre was a gift from Moritoshi Sato (Addgene plasmid # 122960 ; http://n2t.net/addgene:122960 ; RRID:Addgene_122960) " nos3,3'utr: gift from Erez Raz lab

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSDest2_pACre-nos3'utr was a gift from Florence Marlow (Addgene plasmid # 226790 ; http://n2t.net/addgene:226790 ; RRID:Addgene_226790)

  • For your References section:

    Primordial germ cells differentially contribute to the germline in zebrafish. Pahima M, Marlow FL. Development. 2026 Jul 6:dev.204904. doi: 10.1242/dev.204904. 10.1242/dev.204904 PubMed 42403048
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