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p5E-askopos
(Plasmid #226791)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226791 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    p5E-MCS
  • Backbone manufacturer
    Tol2kit v1.0
  • Backbone size w/o insert (bp) 2643
  • Total vector size (bp) 7648
  • Vector type
    Gateway 5'-entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    askopos promoter
  • Alt name
    kop
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    4831
  • Entrez Gene
    kop (a.k.a. zgc:123051)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This construct was cloned by Paloma Bravo. The askopos promoter was cloned from the kop:gfp-nos3,3'utr construct, gifted by the Erez Raz lab (Blaser et al., 2005)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5E-askopos was a gift from Florence Marlow (Addgene plasmid # 226791 ; http://n2t.net/addgene:226791 ; RRID:Addgene_226791)

  • For your References section:

    Primordial germ cells differentially contribute to the germline in zebrafish. Pahima M, Marlow FL. Development. 2026 Jul 6:dev.204904. doi: 10.1242/dev.204904. 10.1242/dev.204904 PubMed 42403048
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