pME-Zebrabow
(Plasmid
#226792)
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PurposeMiddle-entry vector with Zebrabow cassette. For use in Gateway cloning.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 226792 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepME-MCS
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Backbone manufacturerUnknown
- Backbone size w/o insert (bp) 2544
- Total vector size (bp) 6574
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Vector typeGateway cloning middle-entry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameZebrabow
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SpeciesSynthetic
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Insert Size (bp)3809
Resource Information
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A portion of this plasmid was derived from a plasmid made byThis construct was cloned by Maya Pahima. Zebrabow: (Pan et al., Dev. 2013), Addgene plasmid #118221
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME-Zebrabow was a gift from Florence Marlow (Addgene plasmid # 226792 ; http://n2t.net/addgene:226792 ; RRID:Addgene_226792) -
For your References section:
Primordial germ cells differentially contribute to the germline in zebrafish. Pahima M, Marlow FL. Development. 2026 Jul 6:dev.204904. doi: 10.1242/dev.204904. 10.1242/dev.204904 PubMed 42403048