pTol2Dest_nos2:mApple_pBH
(Plasmid #226797)

• Purpose: Transgenic expression of mApple under the nanos2 promoter. Includes a red heart (pBH; cmlc2:RFP) marker.
• Depositing Lab: Florence Marlow
• Publication: Primordial germ cells differentially contribute to the germline in zebrafish. (unpublished)

Backbone

• Vector backbone: pTol2Dest_pBH
• Backbone manufacturer: Florence Marlow, Amanda Heim
• Backbone size w/o insert (bp): 6946
• Total vector size (bp): 10282
• Vector type: Zebrafish expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: nanos2
• Species: D. rerio (zebrafish)
• Insert Size (bp): 2417
• Entrez Gene: nanos2

Cloning Information for Gene/Insert 1

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7

Gene/Insert 2

• Gene/Insert name: emgfp
• Species: Synthetic
• Insert Size (bp): 711
• Promoter: nanos2

Cloning Information for Gene/Insert 2

• Cloning method: Gateway Cloning
• 5′ sequencing primer: EBV

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cloned by Maya Pahima.
pTol2-nos2L:mApple, cmlc2:dsred (pBH) was generated by combining p5E-nos2prom and pME-mApple (Addgene, 195960) (Kemmler et al., 2023) into the pTol2-R4/R2_cmlc2:dsred (pBH) backbone vector (Heim et al., 2014).