pCR-5'Myt1outSB605
(Plasmid #22738)

• Depositing Lab: Lynn Hudson
• Publication: Hudson lab plasmid (unpublished)

Backbone

• Vector backbone: pCR-Blunt II TOPO
• Backbone size w/o insert (bp): 3500
• Vector type: PCR cloning vector
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5 alpha. Standard growing conditons
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Myelin Transcription Factor 1
• Alt name: Myt1
• Species: M. musculus (mouse)
• Insert Size (bp): 605
• Entrez Gene: Myt1 (a.k.a. Nzf2, Nzf2a, Nzf2b, Nztf2)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ cloning site: TOPO (not destroyed)
• 3′ cloning site: TOPO (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7 promoter

Resource Information

• A portion of this plasmid was derived from a plasmid made by: DNA cloned within Hudson lab

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

605bp Myt1 insert can be released with EcoRI. This is the Myt1 5' homology probe. Paper is in preparation. Tentatively titled "The Myelin Transcription Factor 1 (Myt1) is Essential for Neural Development"