PEmaxNG-_2star_IVT
(Plasmid
#227678)
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Purposeplasmid for SpCas9-NG-PEmax** mRNA IVT
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Depositing Lab
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Depositing OrganizationUniversity of Massachusetts, Worcester
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 227678 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 227678-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 227678-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNG-PEmax_2_star
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SpeciesSynthetic
- Promoter T7
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Tag
/ Fusion Protein
- no
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13 Rev
- 3′ sequencing primer polyA-seq
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Due to the difficulties of maintaining and sequencing long homopolymers, Addgene cannot guarantee the exact length of polyA sequence
DNA (Catalog # 227678-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 227678-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PEmaxNG-_2star_IVT was a gift from Scot Wolfe (Addgene plasmid # 227678 ; http://n2t.net/addgene:227678 ; RRID:Addgene_227678) -
For your References section:
Increasing intracellular dNTP levels improves prime editing efficiency. Liu P, Ponnienselvan K, Nyalile T, Oikemus S, Joynt AT, Iyer S, Kelly K, Guo D, Kyawe PP, Vanderleeden E, Redick SD, Huang L, Chen Z, Lee JM, Schiffer CA, Harlan DM, Wang JP, Emerson CP Jr, Lawson ND, Watts JK, Sontheimer EJ, Luban J, Wolfe SA. Nat Biotechnol. 2024 Sep 25. doi: 10.1038/s41587-024-02405-x. 10.1038/s41587-024-02405-x PubMed 39322763
Map uploaded by the depositor.
